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KMID : 0379519990150030369
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한국독성학회지
1999 Volume.15 No. 3 p.369 ~ p.372
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Protective Effect of BR92021, Vi Polysaccharide Typhoid Vaccine, in Mice
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Lim Sang-Min
Jung Hahn-Sun
Lee Youn-Kyeong
Cho Zeung-Keun
Kim Sang-Lin
Lee Yong-Soon
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Abstract
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This study was performed to confirm the immunological efficacy of BR92021, vi polysaccharide typhoid vaccine, in mice. The BR92021 producing strain was less pathogenic than the challenging organism, S. typhi ty2 N4446. BR92021 completely protected the death caused by S. typhi ty2 at above 1.25 ㎍/0.5 ml/dose vaccinized by one time. At the optimally effective dose of 25 ㎍/0.5 ml of BR92021, one immunization was necessary for complete protection against S. typhi ty2-induced death. In potency test, we injected S. typhi ty2 N4446 (LD50=7.9)for the purpose of challenge to immunized mice. When injected to mice, the number of bacteria was about 1,000 CFU/0.5 ml, we observed challenged mouse for 3 days and calculated the ED50 by probits method. It was 0.037 (BR92021 ,lot No. 980001), 0.036 (BR92021, lot No. 980002), 0.032 (BR92021, lot No. 980003) and 0.045 (reference standard) ㎍/0.5 ml/mouse. This result showed that BR92021 vaccine was effective for the protection from desease caused by S. typhi ty2 infection.
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KEYWORD
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BR92021, Vi polysaccharide typhoid vaccine, Immunological efficacy
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